Safranin O-stained antigen microagglutination test for detection of brucella antibodies

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Safranin O-stained antigen microagglutination test for detection of brucella antibodies.

A microagglutination test was compared with a standard tube agglutination test for the detection of brucella antibodies. Advantages of the microagglutination test were that it required less time to perform, had a shorter incubation period, and used less antigen.

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Use of Safranin O for Characterization of Brucella Species.

Moreira-Jacob (J. Bacteriol. 86:599, 1963) reported that 44 strains of Brucella suis were inhibited by safranin 0 in Brucella Agar (Albimi), whereas nearly all of 320 other strains of Brucella were not inhibited. Brinley-Morgan (World Health Organization, Brucellosis document , 246, 1963) examined the growth of more than 80 cultures on serum dextrose agar with added safranin 0, and found that a...

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Effect of centrifugation and microagglutination techniques on Brucella agglutinin titers.

The microagglutination technique without centrifugation was more effective than centrifugation of the standard tube test for increasing Brucella agglutinin titers of specimens with a titer greater than or equal to 160 but was less effective than centrifugation of the standard tube test for specimens with a titer less than 160.

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1981

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.13.2.398-400.1981